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Plant Tissue Culture has evolved as a technology that can be well utilized to solve myriads of crop specific problems. In an era, where climatic changes and land use pattern divergence is causing major hurdles in cultivation pattern of crops, this technique comes as a savior, requiring minimal space for conserving large number of species, thereby providing an alternative for prevention of species from getting endangered and also multiplying large number of plantlets, especially elite genotypes. This method comes handy to scientists and farmers equally, while offering a possibility of entrepreneurship to youngsters, who can develop and adopt this skill based technology. Basically designed for students,as a handbook, it trains and takes them to a travail of applications.