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This timely two-volume reference is the first on this emerging and interdisciplinary topic, covering everything from methods to applications in bioorthogonal chemistry and chemical ligation reactions, focusing on both established as well as recently developed chemistries. In the introduction chapter a brief overview of the field is presented, including a discussion of the origins, importance, and future directions for BO/CS chemistries. The first volume focuses on the details of BO/CS chemistry at the level of fundamental organic chemistry, while the second volume deals with applications, particularly in the areas of chemical biology, biomaterials, and bioanalysis, highlighting the capabilities and potential of BO/CS chemistry. With its three internationally renowned editors each with a different core competence - perfect for an interdisciplinary topic - this of high interest for biochemists, biologists, pharmaceutical chemists and organic chemists.