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This volume presents the proceedings of the 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies. The conference took place in Portoroz, Slovenia, during the week of September 6th to 10th, 2015. The topics of the conference include:§Basics:§Biology of electroporation - in vitro§Biology of electroporation - in vivo§Modelling of electroporation§Molecular dynamics simulations§Electrochemistry§§Electroporation&PEF Technology development:§Large treatment capacities§Laboratory scale or research capacities§Micro and Nanotechnologies§§Electroporation&PEF Applications:§Medical applications (e.g. ECT, IRE, GET, ...)§Veterinary medical applications§Food and biotechnology applications§Environmental applications§Analytical chemistry sampling§Other applications§§Other technologies and applications in Biomedicine, Food and Environment:§Plasma§Ohmic heating§Electrical discharges§Ultrasound§Other technologies§§